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. 2014 Aug 16;42(18):e142. doi: 10.1093/nar/gku733

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Relative and absolute quantifications of ribosomal RNA modifications. (A) Relative quantification displayed as fold changes for several modified nucleosides. The fold changes are the ratio of the modification level of rRNA fragments from WT1 and WT2. Fold changes <1 indicate higher levels in WT2 whereas fold changes >1 indicate higher modification levels in WT1. (B) Absolute modification of modified nucleosides from the same analysis using rRFN values. Am, Gm and Ψ are present in stoichiometrically relevant numbers whereas Cm, I, m⁶₂A and m⁶A are only present in traces.