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. 2014 Aug 16;42(18):e142. doi: 10.1093/nar/gku733

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Reproducibility of rRFN values over 12 weeks. Relative standard deviations (RSDs) in % for the MS/MS signal and the isotope normalized signal of 2’-O-methyladenosine (Am) over a period of 12 weeks. (A) MS/MS abundance of constant amounts and average abundance with RSD. (B) Relative response factor (rRFN) of Am obtained by the measurements shown in (A). (C) RSD of MS/MS signal and rRFN for 10 modified ribonucleosides after 12 weeks. In black, the RSD in % of the MS abundance without ¹³C SIL-IS-based correction is shown. RSDs of the respective rRFN are in green.