Table 1. Limit of detection (LOD) and limit of quantification (LOQ) for 10 modified nucleosides by a dMRM method.

		Current method dMRM		NLS method (20)
Name		LOD (fmol)	LOQ (fmol)	LOD (fmol)
Pseudouridine	Ψ	10	20	100
5-Methyluridine	m5U	10	20	10
Inosine	I	2	5	10
5-Methylcytidine	m5C	1	2	10
6-Dimethyladenosine	m62A	1	2	10
2′-O-Methylguanosine	Gm	0.5	1	10
2-Methylguanosine	m2G	0.5	1	10
2′-O-Methylcytidine	Cm	0.2	0.5	10
1-methyladenosine	m1A	0.1	0.5	100
2′-O-Methyladenosine	Am	0.1	0.5	10
7-Methylguanosine	m7G	0.01	0.05	100

LOD values previously obtained (20) in a neutral loss scan are given for comparison.