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. 2014 Sep 15;42(18):11777–11791. doi: 10.1093/nar/gku805

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — TUTase depletion does not affect miRNA levels. (a) Western blot analysis of Lentivirus-mediated knockdown of Zcchc11 and Zcchc6 in Hela cells. (b) Mature miRNA northern blot of let-7g levels in the indicated transduced Hela cell lines. Valyl tRNA serves as a loading control. (c) Total small RNA from shGFP and shZcchc6/shZcchc11 (shTUT) Hela cells was purified and cloned before sequencing. Each point represents a single miRNA species, and axes are scaled to reads/million (RPM). R² >0.99. (d) The relative number of reads for WT (i.e. non-modified) motif-containing miRNAs is unchanged after TUTase depletion.