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tsEPODs silence activity of the lac promoter. (A) Example of insertion of the reporter cassette within a tsEPOD (grey box). Genes are represented as block arrows, labelled A, B and C. (B) Replacement of tsEPOD sequence with the reporter cassette. (C) Fluorescence output from the reporter cassette from the pitB, yafT, eaeH, yqe loci. On the x-axis, a or b denotes tsEPOD disruption or replacement respectively.
