Figure 2.

Enzyme-dependent δ-elimination process during 5mC excision. (A and B) Radiolabeled 35-mer oligonucleotide duplex containing 5mC at position 18 (25 nM) was reacted with purified MBP-DMEΔ (100 nM) at 37°C for 25 min. Reactions were separated and paused on ice (A) or heat-inactivated at 65°C (B) for 15 min, and then resumed at 37°C for additional 15.6 h. Reactions were terminated at indicated time points and separated on a polyacrylamide gel. (C and D) Reaction products from the experiments in (A and B) were quantitated and plotted over time.