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. 2014 Sep 16;42(18):11502–11516. doi: 10.1093/nar/gku835

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Published by Oxford University Press on behalf of Nucleic Acids Research 2014. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 6. — Origin replication complex is assembled on alphoid DNA monomers. (A, B) ChIP-qPCR with antibodies against Orc2 and Treslin shows enrichment of ORC and Treslin on alpha-satellite monomers. The enrichment was observed with the primers developed from the alpha-satellite consensus sequence. The primers recognize a major fraction of monomers in the human genome. No enrichment was detected with other primers, ChrX-CEN, that recognize a 100 kb centromeric region on the chromosome X. (C, D) ChIP for markers of transcriptionally competent or neutral chromatin and bivalent, repressible chromatin, H3K4me2 and H3K27me3, correspondingly. Precipitated chromatins for all antibodies are normalized with IgG. Student's t-test was used: P < 0.01 (*), ±SD.