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. 2014 Sep 22;42(18):11419–11432. doi: 10.1093/nar/gku842

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Tls1 is specifically required for telomeric heterochromatin assembly. (A and D) Serial dilution analyses to measure the expression of ura4⁺ reporter genes and sensitivity to TBZ. (B and E) ChIP analyses of H3K9me2 levels at ura4⁺ reporter genes, normalized to act1 gene. Error bars represent standard deviation of three experiments. (C) qRT-PCR analyses of transcripts derived from repeat elements, tlh1 for subtelomeric region, dh for pericentric region and cenH for mating type locus, normalized to act1. Wild-type levels were set to 1. Error bars represent standard deviation of three experiments.