Figure 4.

Tls1 associates with the spliceosome. (A) Flag-Tls1 is functional in telomere silencing as indicated by serial dilution analysis to measure the expression of TEL::ura4⁺. (B) Flag-Tls1 is functional in telomere length control as indicated by Southern blot analysis with a telomere probe. (C) Mass spectrometry analyses of purified Flag-Tls1 complex. (D) Co-immunoprecipitation analysis of Flag-Tls1 and Brr2-myc. The whole cell extracts were incubated with Flag-agarose beads to pull down Flag-tagged Tls1. Flag and myc antibodies were used to perform western blot analyses. (E) Tls1 interacts with Brr2 in a yeast two-hybrid assay. Tls1 was fused with Gal4 DNA-binding domain and Brr2 was fused with an activation domain. Interaction between Tls1 and Brr2 resulted in the activation of a HIS3 reporter gene, allowing cells to grow in the absence of histidine.