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. 2014 Sep 22;42(18):11419–11432. doi: 10.1093/nar/gku842

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Tls1 regulates the proper splicing of shelterin components. (A) List of number of introns present in the genes of telomere proteins. (B) RT-PCR analyses of RNA with primers flanking introns. Genomic DNA (gDNA) was used in a control PCR reaction to indicate the position of unspliced form. The structures of the genes are indicated on top and boxes represent exons. The primer positions were labeled. (C) qRT-PCR analyses of intron retention levels, normalized to act1. Wild-type levels were set to 1. Bars represent PCR fragment amplified. (D) Western blot analyses of shelterin protein levels. Ponceau S staining was used as the loading control. (E) Live cell imaging of cells expressing GFP-tagged versions of shelterin. Scale bar, 1 μm.