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. 2014 Sep 22;42(18):11419–11432. doi: 10.1093/nar/gku842

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Introducing cDNAs of shelterin is sufficient to restore telomeric heterochromatin and proper telomere length in tls1Δ cells. (A) Western blot analysis of shelterin protein levels. Asterisk indicates a non-specific band. (B) Serial dilution analyses to measure TEL::ura4⁺ reporter gene expression. (C) ChIP analyses of H3K9me2 levels at TEL::ura4⁺, normalized to act1. Error bars represent standard deviation of three experiments. (D) Southern blot analyses of telomere length with a telomere probe.