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. 2014 Sep 22;42(18):11528–11545. doi: 10.1093/nar/gku856

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — smFRET measurements on pd-12ThGQ-5′ construct at 150 mM K⁺ and different ATP concentrations. The BLM concentration is maintained at 1 μM during ATP titration. (A) The gray shaded histogram shows the folded state, while the histogram with black, red, green and blue outlines represent 0, 4, 20 and 100 μM ATP, respectively. The unfolded GQ population is represented by E_FRET ≤ 0.40, and is highlighted by the orange rectangle. The peak at E_FRET ≈ 0.50 in black histogram represents the BLM-bound folded GQ state. The inset shows a schematic of the DNA construct used for these measurements. (B) The total unfolded population in these steady-state histograms, as obtained by integrating the area at E_FRET ≤ 0.40, decreases as ATP concentration is increased. This decrease is attributed to more frequent dissociation of BLM from the DNA substrate.