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. 2014 Sep 22;42(18):11528–11545. doi: 10.1093/nar/gku856

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — smFRET measurements on pd-polyT15 (pdDNA with 15 thymine long 3′ overhang) at 150 mM K⁺. BLM binding to ssDNA representing the overhang in the GQ substrate depends on the nucleotide state. (A) pd-polyT15 is in coiled conformation with E_FRET ≈ 0.85 before BLM is added. (B)–(D) BLM binding to the overhang in different nucleotide states at 1 μM BLM. (B) Nt-free state. (C) 1 mM ATPγS. (D) 1 mM ADP. Binding is most efficient in the ATPγS state, followed by the nt-free state and then the ADP state.