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. 2014 Sep 22;42(18):11528–11545. doi: 10.1093/nar/gku856

Figure 5.

Figure 5.

Bar diagrams illustrating the overhang length dependence of BLM-mediated GQ unfolding in the nt-free (light gray) and 1 mM ATPγS states (dark gray). All measurements were carried out at 150 mM K+, and 1 μM BLM concentration. Overhangs of 2, 4, 6, 8, 10, 12 and 15 nt were studied. The unfolded populations were determined by subtracting the folded state at 150 mM K+ for each construct from the respective nucleotide state with 1 μM BLM concentration. The error bars were obtained based on the variation in BLM-mediated GQ unfolding activity for different data sets.