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smFRET histograms showing BLM-mediated GQ unfolding of pd-hGQ12T-5′. This construct has a 12 thymine long 5′ overhang. (A) BLM cannot unfold this construct at 150 mM K+ in either nt-free or ATPγS states, indicating orientation of binding on the overhang is important. (B) However, if the GQ stability is lowered at 50 mM K+, significant BLM-mediated GQ unfolding occurs in both nt-free and ATPγS states.
