Figure 8.

A comparison of BLM-mediated GQ unfolding in 150 mM K⁺ and 150 mM Na⁺, in which pd-hGQ12T folds into different conformations. (A) The green shaded histogram represents the folded GQ state in 150 mM Na⁺, and the brown histogram represents the folded state in 150 mM K⁺. The histogram in Na⁺ is narrower and can be fit by a single Gaussian peak, indicative of a single conformation, while that for K⁺ is broader and requires two Gaussian peaks for fitting (shown by black and red dashed curves), indicative of multiple folding conformations. (B) and (C) BLM-mediated GQ unfolding in 150 mM Na⁺ in 300 and 1000 nM BLM, respectively. (D) A comparison of BLM-mediated GQ unfolding in 150 mM Na⁺ and 150 mM K⁺ for 300 and 1000 nM BLM. These data show that unfolded GQ population is at similar levels in both ionic conditions, suggesting that BLM unfolds different conformations at similar levels.