Table 1. The DNA constructs used in these studies.
| Name | Sequence |
|---|---|
| hGQxT | 5′- TGG CGA CGG CAG CGA GGC TAG GGT TAG GGT TAG GGT TAG GG (xT) Cy3 |
| x = 2, 6, 8, 10, 12, or 15 | |
| hGQ4nt | 5′- TGG CGA CGG CAG CGA GGC TAG GGT TAG GGT TAG GGT TAG GG TTAG Cy3 |
| 3Ly1Lp12T | 5′- TGG CGA CGG CAG CGA GGC TTG GGT GGG TGG GTG GG(12T) Cy3 |
| hGQ12T-5′ | 5′-Cy5-(12T) GGG TTA GGG TTA GGG TTA GGG TT TGG CGA CGG CAG CGA GGC |
| 12ThGQ-5′ | 5′-Cy3-TT GGG TTA GGG TTA GGG TTA GGG(12T) TGG CGA CGG CAG CGA GGC |
| polyT15 | 5′- TGG CGA CGG CAG CGA GGC (15T) Cy3 |
| polyT15–5′ | 5′-Cy5-(15T) TGG CGA CGG CAG CGA GGC |
| polyT35 | 5′-TGG CGA CGG CAG CGA GGC (35T) Cy3 |
| RNA-Stem | 5′- Cy5 GCC UCG CUG CCG UCG CCA Biotin |
| DNA-Stem | 5′- Biotin GCC TCG CTG CCG TCG CCA Cy3 |
The constructs whose names are in bold letters are hybridized with the RNA-Stem to form a pdDNA. polyT15–5′ and DNA-Stem are hybridized to form pd-polyT15–5′. The underlined nucleotides form the GQ structure. The number of consecutive thymines are written in parenthesis, e.g. (12T) means 12 consecutive thymines.