Table 2. A summary of Langmuir binding analysis performed for pd-hGQ12T.

Summary of Langmuir Isotherm fits y = αx/(x + Keq)
State	K+ Con.	Effector	Reference state	α (%)	Keq	Figure
Nt-free	150 mM	BLM	Folded state	49 ± 1	305 ± 16 nM BLM	Figure 1E
1 mM ATPγS	150 mM	BLM	Folded state	53 ± 3	108 ± 24 nM BLM	Figure 1I
1 mM ADP	150 mM	BLM	Folded state	32 ± 1	55 ± 5 nM BLM	Figure 1M
1 μM BLM	150 mM	ATPγS	1 μM BLM, Nt-free	30 ± 4	4.2 ± 1.1 μM ATPγS	Figure 2D
1 μM BLM	150 mM	ADP	1 μM BLM, Nt-free	14 ± 1	1.2 ± 0.1 μM ADP	Figure 2H
1 μM BLM	150 mM	AMP-PNP	1 μM BLM, Nt-free	18 ± 1	2.1 ± 0.3 AMP-PNP	Figure S11

All assays include 5 mM Mg⁺⁺ in addition to the indicated K⁺ concentration. Folded state refers to the state in the presence of the indicated K⁺ concentration but in the absence of BLM. In the titrations where ATPγS or ADP is the effector, the reference state is selected as the nt-free state in the presence of BLM (at the indicated concentration) to reflect the influence of nucleotide on BLM-mediated GQ unfolding. Note that isotherm fit parameters cannot be compared if the reference state is different. Also, note that the fit parameters for ADP titration at 1 μM BLM, last row of table, represent relative folding rather than unfolding.