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Analysis of malDOTA conjugation to anti-ALCAM-8L, -5L and -3L cDbs. (A) SDS–PAGE of anti-ALCAM-8L, -5L and -3L cDbs post-malDOTA conjugation. (B) Size exclusion chromatography analysis at 280 nm using the Superdex 75 column at 0.5 ml/min of malDOTA-conjugated anti-ALCAM-8L, -5L, and -3L cDbs using PBS as a running buffer. Reference arrows indicate albumin (66 kDa) at 20.8 min, carbonic anhydrase (29 kDa) at 24.7 min and cytochrome C (12.4 kDa) at 27.4 min.
