Table I.
Elution times of unconjugated anti-ALCAM-8L, 5L, and 3L cDbs in non-reducing conditions and reducing conditions, and mal488- and malDOTA-conjugated anti-ALCAM-8L, 5L and 3L cDbs in non-reducing conditions using Superdex 75 size exclusion chromatography. Elution times are shown in minutes and numbers in parentheses indicate the percentage at each retention time if applicable. Note the reference elution times are as follows: albumin (66 kDa) at 20.8 min, carbonic anhydrase (29 kDa) at 24.7 min and cytochrome C (12.4 kDa) at 27.4 min
| Non-reducing |
Reducing |
Maleimide-488 |
Maleimide-DOTA |
|||||
|---|---|---|---|---|---|---|---|---|
| Construct | cDb | scFv | cDb | scFv | cDb | scFv | cDb | scFv |
| ALCAM-8L | 22.80 (82%) | 25.10 (18%) | n/a | 24.95 | 22.85 (51%) | 24.80 (49%) | n/a | 24.32 |
| ALCAM-5L | 22.98 | n/a | 23.31 | n/a | 22.91 | n/a | 22.86 | n/a |
| ALCAM-3L | 23.02 | n/a | 23.11 | n/a | 22.85 | n/a | 22.70 | n/a |