Table II.
Mass spectrometry review of intact unconjugated anti-ALCAM-3L cDb, anti-ALCAM-3L-amide-DOTA-cDb and anti-ALCAM-3L-thioether-DOTA-cDb. Expected addition of one amide-DOTA conjugation is 386.2 kDa and one thioether-DOTA is 526.2 kDa
| Modification | Expected (kDa) | Found (kDa) | Difference (kDa) | |
|---|---|---|---|---|
| Unconjugated | n/a | 50344.2 | 50341.6 | −2.6 |
| NHS-DOTA | 0× DOTA | 50344.2 | 50342.3 | −1.9 |
| 1× DOTA | 50730.4 | 50729.3 | −1.1 | |
| 2× DOTA | 51116.8 | 51115.2 | −1.4 | |
| 3× DOTA | 51502.8 | 51501.3 | −1.5 | |
| 4× DOTA | 51889.0 | 51888.1 | −0.9 | |
| 5× DOTA | 52275.2 | 52275.5 | +0.3 | |
| malDOTA | 0× DOTA | 25173.1 | 25138.2 | −34.9 |
| 1× DOTA | 25699.3 | 25716.8 | +17.5 |