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. 2014 Oct 9;8:112. doi: 10.3389/fnana.2014.00112

Figure 3.

Figure 3

Representative fluorescence photomicrographs showing FG- (A, green), NT- (B, red), and NTR2- (C, blue) triple-labeled neurons in the RVM. The images from (A–C) are merged in (D). The rectangular areas in (A–D) were enlarged and displayed in (A’–D’). The arrows indicate NTR2/NT double-labeled neurons; the unfilled arrowheads indicate NTR2-IR neurons; and the filled arrowheads indicate FG/NT/NTR2 triple-labeled neurons in the RVM (D’). Scale bar = 80 µm (A–D) or 30 µm (A’–D’). (E) The mean number of NTR2-IR or NTR2/NT double-labeled or NTR2/NT/FG triple-labeled neurons in the RVM at seven rostro-caudal levels (from 11.4 mm to 9.96 mm caudal to bregma) revealed via immunofluorescence histochemical triple-staining for NTR2, NT, and FG (n = 5 rats).