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. 2014 Oct 9;8:112. doi: 10.3389/fnana.2014.00112

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Copyright © 2014 Wang, Zhang, Feng, Meng, Wu, Wang, Li, Zhang, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electron photomicrographs showing the synaptic connections in the RVM revealed using the triple-labeling method. An NT-IR axon terminal (Ax-NT) (A) or a BDA-labeled axon terminal (Ax-BDA) (B) synapses on a dendrite that is retrogradely labeled with HRP (Den-HRP) and immunopositively stained for NTR2. The synaptic contact between an NT/BDA double-labeled axon terminal (Ax-BDA+NT) and an HRP-labeled dendritic profile (Den-HRP) is also shown (C). The arrowheads indicate the post-synaptic membranes. Mit: mitochondria.