Skip to main content
. 2014 Sep 11;57(19):7916–7932. doi: 10.1021/jm5009902

Figure 3.

Figure 3

(A) Representative coimmunoprecipitation (IP) of PIM1 and GBP1 in SKOV3 cell line treated with NSC756093 (3 h at 100 nM). The signal was revealed using anti-GBP1 antibody detected as a specific band of 67 kD. Lane 1: input of SKOV3 lysate. Lane 2: flow through co-IP. Lane 3: flow through control. Lane 4: co-IP. PIM1:GBP1 (antibody anti-PIM1). Lane 5: co-IP. Negative control (antibody anti-IGg). The presence of the signal in lane 4 means no interference in the GBP1:PIM1 binding in DMSO and the inactive NSC756090; the absence of detectable signal in the presence of NSC756093 means that the compound is able of inhibiting the GBP1:PIM1 interaction. (B) Bar chart showing the densitometric analysis of the experiment shown in A, performed in two independent experiments. A significant (double asterisks = p < 0.001, Anova) suppression of the co-IP was noticed in both experiments only with the compound NSC756093.