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. 2014 Sep 4;5(10):1156–1161. doi: 10.1021/ml500297n

Figure 4.

Figure 4

Inhibition of CYP2B6 bupropion hydroxylase activity by EFV and EFV analogues. CYP2B6 (50 nM) was incubated with bupropion (40 μM) and EFV or EFV analogue (10 μM) in 100 mM potassium phosphate buffer for 10 min at 37 °C. Hydroxybupropion formation was analyzed using uHPLC–MS/MS. Inhibition is reported as a percentage of hydroxybupropion formation in the presence of a vehicle control (no EFV or EFV analogue present). Data reflect the mean ± SD of three replicate experiments performed in duplicate. Symbols for statistical significance represent comparisons to vehicle control (*) or EFV (#). Two symbols, p ≤ 0.01; three symbols, p ≤ 0.001.