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. Author manuscript; available in PMC: 2014 Oct 9.
Published in final edited form as: Nat Neurosci. 2013 Oct 6;16(11):1576–1587. doi: 10.1038/nn.3541

Figure 3.

Figure 3

Most OSVZ stem cells in the ganglionic eminences lack M phase fibers and are randomly oriented. (a) In the human ganglionic eminences, nearly all OSVZ progenitors in M phase (labeled by 4A4 antibody for p-vim) displayed a simple, rounded morphology, indicating that any glial fibers were retracted during M phase. This was true for both neural stem cells (SOX2+ DLX2−, white arrowheads) and intermediate progenitor cells (DLX2+, orange arrowheads). (b) The few progenitors in the MGE OSVZ that maintained short glial fibers during M phase were unipolar, expressed SOX2 and nestin, and had random orientation. Left, SOX2 stains of ganglionic eminences in frontal PCW14 sections. Second column, magnified images of areas boxed in red, with the overall contour of nestin fibers depicted by orange arrows. Right, magnification of the areas boxed in white showing features of 4A4+ cells that retained fibers during M phase, with cell bodies marked by white arrowheads and orientation of unipolar fibers marked by green arrows. Only one example (second row) was aligned with the overall glial fiber scaffold.