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. 2014 Sep 15;111(39):14217–14222. doi: 10.1073/pnas.1409653111

Fig. 1.

Fig. 1.

IDH1R132H inhibits growth of murine glioma progenitor cells. (A) Incidence of glioma formation with p53 +/− or p53 −/− neural stem cultures expressing PDGF-B or human IDH1R132H (hIDH1R132H). ND, not determined. (B) CyQuant assay of glioma progenitors (line 5 in A) expressing either no exogenous protein (control), human IDH1R132H (hMUT), human IDH1WT (hWT), shRNA to murine IDH1 (KD), or hMUT and hWT (hMUT + hWT). For B and C, bars represent mean ± SEM for three or more determinations; *P < 0.005, **P < 0.0005 vs. control, t test. (C) (Top) Clone formation assay. mMUT, a clonal line expressing murine IDH1R132H; mMUT + mWT, mMUT clonal line rescued with IDH1WT/RCAS. (Middle) 2-HG concentrations. +/−, basal levels; ++, three- to fivefold; +++, 5- to 10-fold; ++++, 10- to 50-fold, relative to control (see Fig. S1). (Bottom) Western blots for IDH1WT and IDH1R132H.