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. 2014 Sep 15;111(39):14159–14164. doi: 10.1073/pnas.1414394111

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Fig. 4. — Characteristics of tRNA-derived sRNAs. (A) tasRNA undergoes a larger expression change than endo-siRNAs in the Giardia differentiation process. (B) The length distribution of tasRNAs in four sRNA libraries. (C) The composition of tasRNAs in four sRNA libraries. (D) Northern blot analysis of nine tasRNAs derived from three tRNAs. (E, Upper) Northern blot analysis of 5EsRNA. E, Lower indicates the distribution of sRNA across pseudotRNA-Gln (TTG). (F) Two cleavages in mature tRNAs. (G) Five kinds of sRNAs derived from mature tRNAs. Trop refers to trophozoite. E3h, E6h, E12h, E24h, and E48h refer to encystation 3, 6, 12, 24, and 48, respectively. 3tasRNA, tRNA 3′ end-associated sRNA.