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. 2014 Sep 15;111(39):14181–14186. doi: 10.1073/pnas.1415690111

Fig. 6.

Fig. 6.

Ribosome binding of ACTIN, JIP23, THIONIN, RBCS, and LHCB2 transcripts in rabbit reticulocyte lysates supplemented with JIP60, RIP-UP, RIP30, and eIF4E. (A) Synthesis of ACTIN, JIP23, and THIONIN proteins in JIP60- (a), RIP-UP– (b), RIP30- (c), and eIF4E-incubated (d) reticulocyte lysate. Fraction 1 obtained after sucrose gradient centrifugation contains the 40S ribosomal subunit, fraction 2 contains the 60S ribosomal subunit, and fractions 3–8 contain cytoplasmic polysomes of increasing size. Protein was detected by Western blotting using respective monospecific antisera. Note that, to detect minor traces of polysome-bound messengers, 50-fold–higher levels of RNP material were loaded onto the sucrose gradient in a compared with b–d. (B) Synthesis of RBCS and LHCB2 in eIF4-incubated (a) and mock-incubated (b) reticulocyte lysates, as assessed by Western blotting.