Skip to main content
. 2014 Aug 13;111(39):14147–14152. doi: 10.1073/pnas.1404171111

Fig. 1.

Fig. 1.

ADI-PEG20 induced both caspase-independent cell death and DNA leakage. (A) CWR22Rv1 cells were treated with ADI-PEG20 and stained with propidium iodide (PI). The percentage of hypodiploid cells began to rise at 48 h and significantly increased after 72 h. (B) Western blotting showing the lack of caspase 7, 8, and 9 activation in cells treated with ADI-PEG20. (C) DAPI staining reveals nuclear DNA before leakage at 24 h (early time points) and after leakage at 96 h and 120 h (late time points) posttreatment. (D) The same samples were stained with another fluorescent DNA dye, DRAQ5. Cells were stained with plasma membrane marker E-cadherin to reveal its boundary. Cells were treated with UV or Taxol (1 nM) to demonstrate the appearance of apoptotic body and mitotic catastrophe, respectively. Rapamycin (2 μM) treatment did not produce the same effect. (E) Prolong incubation with arginine-depleted medium also induced the same phenotype. (F) The bar graph illustrates the positive increment of leaked DNA particles in a population of cells showing the phenotype.