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. 2014 Sep 15;111(39):14289–14294. doi: 10.1073/pnas.1407640111

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Fig. 4. — Inguinal depot-selective modulation of Foxa3 levels via adenoviral delivery affects thermogenic gene programs and browning. (A) H&E and UCP1 staining of inguinal fat tissues of 2-mo-old mice injected bilaterally with adenoviruses expressing control (Ad-Ctrl) or Foxa3 (Ad-Foxa3). (B) mRNA levels of thermogenic and brown fat genes in iWAT expressing control (Ad-Ctrl) or Foxa3 adenovirus (Ad-Foxa3). (C) H&E and UCP1 staining of inguinal fat tissues of 8-mo-old mice injected bilaterally with control (Ad-U6) or shFoxa3 (Ad-shFoxa3) adenoviruses. (D) mRNA levels of thermogenic and brown fat genes in iWAT expressing control (Ad-U6) or adenoviral shFoxa3 (Ad-shFoxa3). n = 6 per group. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 compared with controls.