Fig. 4.
Survival of 4 dpf fish treated with either MSO or NMDA receptor antagonists and then exposed to NH4Ac. Zebrafish larvae were treated with either 10 μM or 30 μM MSO (A and E), MK-801 (B and F), memantine (C and G) and ketamine (D and H) for 20 min followed by addition of either 4 mM NH4Ac. Fish were monitored for heartbeats every 20–30 min. Solid black line — survival of fish in 4 mM NH4Ac; dashed gray line — survival in 10 μM drug and 4 mM NH4Ac; solid gray line — survival in 30 μM drug and 4 mM NH4Ac. Survival curves represent one of two biological replicates with similar results.
