Fig. 5.

Survival of 4 dpf zebrafish treated with either MSO or MK-801 or both drugs and exposed to NH₄Ac. A. 4 dpf zebrafish were treated with 10 μM MK-801 (dashed dark gray line), 10 μM MSO (dashed light gray line) or 10 μM MK-801 and 10 μM MSO (solid gray line) for 20 min followed by addition of 4 mM NH₄Ac. Control fish were treated with NH₄Ac alone (solid black line). Fish were monitored for the presence of heartbeat every 20–30 min. B. 4 dpf zebrafish were treated with 30 μM MK-801 (dashed dark gray line), 10 μM MSO (dashed light gray line) or 30 μM MK-801 and 10 μM MSO (solid gray line) for 20 min followed by addition of 4 mM NH₄Ac. Control fish were treated with NH₄Ac alone (solid black line). Fish were monitored for heartbeats every 20–30 min. Survival curves represent one of two biological replicates with similar results.