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. 2014 Oct 9;10(10):e1004672. doi: 10.1371/journal.pgen.1004672

Figure 4. Plk1 is important for cleavage of the Scc1 sensor.

Figure 4

(A) HeLa cells were incubated with 5 µM STLC and with or without 100 nM BI2536 for 1 h. Cells were driven out of mitosis with 4 µM of Cdk1 inhibitor RO-3306 in order to observe the activity of separase. Single Z-plane images were depicted. Size bars: 10 µm. (B) Quantification of (A). Separase activity decreases by 50% upon Plk1 inhibition although the cells exited mitosis. n≥10. Error bars represent SD. (C) Chromosome condensation of cells from (A) after 168 min incubation with Cdk1 inhibitor. Upper panel shows the Z-plane where the centrosomes were in focus, and the lower plane shows the Z-plane where the nucleus was in focus. Note that a fraction of the mCherry-Scc1(142-467)-ΔNLS-GFP-PACT sensor accumulates inside the nucleus. Scale bar: 10 µm.