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. 2014 Oct 9;10(10):e1003877. doi: 10.1371/journal.pcbi.1003877

Figure 1. Knock-down of CTCF and Rad21.

Figure 1

The nuclear fraction of control cells (04-147) and cells depleted of CTCF, Rad21 or both simultaneously were analysed by SDS-PAGE followed by immunoblotting. β-Actin was used as a control when calculating changes in CTCF and Rad21 protein levels after siRNA-mediated knock down. Average protein amounts of at least three independent experiments are shown. Error bars represent standard deviation.