Table 3.
Densitometric analysis of the silver stained SDS-PAGE gel following PICUP at pH 7.5 using Image J. The area of each band in a particular lane was normalized by division by the sum of the areas of all the bands in that particular lane. This quotient was multiplied by 100 to yield the percentages in the table. Oligomer order 1–7 in the table refers to monomer through heptamer.
|
Oligomer Order (n) |
Aβ42 | iAβ42 | Ac-iAβ42a | |||
|---|---|---|---|---|---|---|
| 0h | 26h | 0h | 26h | 0h | 26h | |
| 1 | 19.4 | 21.2 | 23.1 | 22 | 2.1 | 3.5 |
| 2 | 13.1 | 13.4 | 25 | 25.6 | 38.4 | 49.1 |
| 2+ | NP | NP | NP | NP | 38.4 | 42.7 |
| 3 | 5.9 | 7.3 | 7.8 | 12.7 | 13.2 | 2.6 |
| 4 | 20.6 | 19.4 | 12.4 | 11.3 | 3.5 | NP |
| 5 | 21.9 | 20.8 | 18.3 | 15 | NP | NP |
| 6 | 14.7 | 14.7 | 11.3 | 11 | NP | NP |
| 7 | 4.2 | 3.2 | 2.0 | 2.4 | NP | NP |
a
1= <3.5 kDa, 2= runs as if it were dimer, 3= between dimer and trimer, 4= > trimer.
*
= bands not quantified (see main text). NP refers to Not Present.