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. 2014 Oct 9;10(10):e1004682. doi: 10.1371/journal.pgen.1004682

Figure 4. Impaired function of DNA polymerase α results in telomere deprotection.

Figure 4

(A) Frequency of anaphases with bridges in floral tissues. (B,C) Amplification of chromosome end-to-end fusions by PCR in pol α and icu2-1 mutants. Two combinations of subtelomeric primers specific for different chromosome arms (3L+1R and 2R+1L) were used. PCR products were detected by Southern hybridization with a telomeric probe. (D) Increased level of t-circles in pol αand icu2-1 mutants measured by TCA. Signals from t-circles (arrowhead), TRFs, and interstitial telomeric DNA (asterisks) are indicated.