Table 2. F and P values from the two-factor ANOVA comparing percentages of individual leaf extract salicinoids from Populus tremula trees of four chemotypes (CN, AC, CN-AC, and TL), grown in two environments (greenhouse and field), and their interaction (E*Ct).

Compound	Environment		Chemotype		E*Ct
	df = 1		df = 3		df = 3
	F	P	F	P	F	P
Salicortin	17.0	<0.001	90.1	<0.001	8.2	<0.001
Tremulacin	60.5	<0.001	436.8	<0.001	3.8	0.011
Salicin	0.4	0.508	10.0	<0.001	0.8	0.497
Tremuloidin	22.6	<0.001	71.8	<0.001	3.8	0.010
HCH-salicortin	10.9	0.001	22.6	<0.001	2.3	0.081
HCH-tremulacin	25.8	<0.001	67.2	<0.001	1.1	0.335
Salicyloylsalicin	26.9	<0.001	14.8	<0.001	2.4	0.066
Acetyltremulacin	22.0	<0.001	35.2	<0.001	1.5	0.225
2'-(Z)-Cinnamoylsalicortin	4.9	0.028	28.5	<0.001	4.5	0.004
2'-(E)-Cinnamoylsalicortin	0.2	0.645	456.4	<0.001	0.9	0.458
Cinnamoylsalicortin (I1)	17.4	<0.001	282.1	<0.001	6.0	0.001
Cinnamoylsalicortin (I2)	178.2	<0.001	317.2	<0.001	288.7	<0.001
Acetylcinnamoylsalicortin (I1)	14.9	0.000	21.3	<0.001	16.7	<0.001
Acetylcinnamoylsalicortin (I2)	17.4	<0.001	38.7	<0.001	30.5	<0.001
HCH-cinnamoylsalicortin (I1)	7.6	0.006	607.4	<0.001	21.9	<0.001
HCH-cinnamoylsalicortin (I2)	3.2	0.073	855.5	<0.001	2.7	0.048
2'-Acetylsalcortin	17.0	<0.001	90.1	<0.001	8.2	<0.001
2'-Acetylsalicin	2.4	0.126	123.5	<0.001	1.5	0.223
Lasiandrin	18.8	<0.001	27.2	<0.001	2.7	0.048

I1 and I2 indicate isomers 1 and 2, respectively, designated by UHPLC retention times. See Figure 3 for corresponding data.