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. 2014 Oct 9;9(10):e108745. doi: 10.1371/journal.pone.0108745

Figure 4. Tissue specific cyanogenic glucoside biosynthetic gene expression in Z. filipendulae larvae.

Figure 4

Tissue localization of CYP405A2, CYP332A3 and UGT33A1 in a representative larva determined by in tube in situ PCR analysis on 80 µm transverse sections using tetramethylrhodamine isothiocyanate (TRITC) and analyzed using fluorescence microscopy. A–D) Controls to visualize the different cell types analyzed using light microscopy, with 4.7 ms exposure time. E) Negative control excluding primers in the PCR reaction, visualized with red light excitation and 370.4 ms exposure time. F) Negative control visualized with red light excitation and 960.8 ms exposure time. G–I) Expression of CNglc biosynthetic genes as monitored by TRITC labeling, visualized with red light excitation and 960.8 ms exposure time. J) Expression of UGT33A1 as monitored by TRITC labeling, visualized with red light excitation and at 370.4 ms exposure time. br, basal ring; cII, small (type II) cuticular cavity; ep, epidermis; fb, fat body; hf, hair follicle; lc, lamellate cuticle; sc, sensory cell; and sh, sensory hair (seta). Scale bars: 100 µm.