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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Nat Med. 2014 Sep 7;20(10):1174–1181. doi: 10.1038/nm.3655

Figure 3.

Figure 3

Inhibition of JAK/STAT signaling rescues the proliferation defect of satellite cells from older adult muscle. (a) Effect of siRNA knockdown of Jak2 or Stat3 on the symmetric divisions of satellite stem cells (Pax7+/Myf5−) on myofibers from Myf5Cre; R26R-YFP cultured for 42h in vitro. (b) Quantification of the total number of dividing satellite cells on single fibers cultured for 42 h in vitro following siRNA knockdown of Jak2 or Stat3. (c) Quantification of the average number of Pax7+ satellite cells per fiber for each age stage following siRNA knockdown. (d) Quantification of the percentage of committed satellite cells (Pax7−/MyoD+) present on single myofibers following 72 h culture following treatment with siRNAs against Stat3 or Jak2. (e) Quantification of symmetric satellite stem cell divisions following JAK/STAT inhibitor treatment for 42 h on myofibers from young adult mice. (f) Quantification of the total number of dividing satellite cells on single fibers cultured for 42 h in vitro following pharmacological inhibition of Jak2 or Stat3. (g) Quantification of the average number of Pax7+ satellite cells per fiber for each age stage following treatment with small molecules. (h) Quantification of the percentage of committed satellite cells (Pax7−/MyoD+) present on single fibers following 72 h culture in the presence of JAK/STAT inhibitors. Data represent the mean ± SEM conducted in at least biological triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001.