Figure 2. Effect of CsA-mediated apoptotic cell death on p53 expression levels.

GH3 cells were incubated in DMEM with or without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. p53 expression (A) was determined by Western blotting and the relative amount (B) was calculated as described in the Materials and Methods. *p<0.05, **p<0.01 vs. serum treatment. ^# p<0.05, ^### p<0.001 vs. no serum treatment.