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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Hypertens Pregnancy. 2014 Jul 2;33(4):412–426. doi: 10.3109/10641955.2014.925564

Figure 3. Effect of Sam68 expression levels on sFlt-1 VEGFR1 isoform splicing.

Figure 3

A. Sam68 and control GAPDH protein levels in triplicate control (left) and anti-Sam68 siRNA-expressing HEK293 cells. B. Quantification of of Sam68 protein in samples shown in A. shRNA induction results in approximately 40% relative reduction of Sam68 protein. C–D: Reduction of Sam68 protein by shRNA correlates with 25% loss of sFlt-1 VEGFR1 mRNA isoform relative to control β-actin (C) and 20% loss of sFlt-1 isoform relative to Exon12-13 spliced VEGFR1 isoforms (D). Statistical significance of expression changes are shown above as p values calculated by Students two-tailed t-test. **highly significant (p ≤ 0.02)