(a) Whole-mount image of the external ear of E15.5
p53−/− embryo (right) and control
embryo (left) showing normal ear pinna development. (b) Whole mount
image of E13.5 p53−/− embryo (right) and
control embryo (left) showing normal retinal development and no evidence of
coloboma. (c) Whole-mount image of E15.5
p53−/− embryo (right) and control
embryo (left) with normal lower jaw development. (d) Alizarin Red
(bone) and Alcian Blue (cartilage) whole-mount stained E14.5
p53−/− embryo (right) showing normal
long bone formation of the ulna (u), humerus (h), mandible (m), and femur (f)
relative to control littermate (left). Bottom: Quantification of bone lengths shown
as percent of E14.5 littermate controls (n=3). (e) Representative
images of H&E-stained sagittal sections of E13.5 control (left) and
p53−/− hearts (right) showing all
three cardiac cell types in both genotypes. en: endocardium; ep: epicardium; myo:
myocardium (arrows). (f) Analysis of H&E-stained transverse
sections of E13.5 p53−/− and control
hearts revealing normal outflow tract development. (Top) The main pulmonary artery
(MPA) and aorta (Ao) are fully septated, and the MPA connects to the right ventricle
(RV) in p53−/− hearts. (Bottom) The aorta
connects to the left ventricle (LV). Φventricular outflow tract
that connects the left ventricle and aorta. PV: pulmonary valve, AV: aortic valve
(g) Analysis of transverse sections of H&E-stained E13.5
p53−/− hearts (right) reveals normal
atrioventricular cushions which have remodeled to form mature, elongated mitral (mv,
arrowhead) and tricuspid (tv, arrow) valves similar to control hearts (left). RA:
right atrium; LA: left atrium; RV: right ventricle; LV: left ventricle.
(h) H&E-stained sagittal section of kidney from
p53−/− (right) and control embryos
(left) showing normal renal size and development. (i) H&E-stained
transverse section of thymi in p53−/−
E13.5 embryo (right) reveals similar thymus size compared to control littermate
(left).