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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Hypertension. 2014 Sep 2;64(5):1032–1039. doi: 10.1161/HYPERTENSIONAHA.114.03900

Figure 4.

Figure 4

Adhesion molecule expression and angiotensin (Ang) II-induced monocyte/endothelial adhesion. (A) Expression of vascular cell adhesion molecule-1 (VCAM-1) on the endothelium of the different bone marrow (BM)-transplanted animals after Ang II infusion. (B) Cardiac VCAM-1 mRNA in the same animals. SVEC4–10 cells were transfected with control or Bcl10 siRNA and were treated with Ang II as described in the methods. (C) Endothelial cell lysates were also analyzed for Bcl10 knockdown by Western blotting and showed nearly complete Bcl10 knockdown. WEHI-274.1 mouse monocytes were added, washed and counted as described. (D) Representative bright field micrographs of individual 20x fields. (E) Fold increase in monocyte adherence upon Ang II treatment in Bcl10 and control siRNA treated endothelial cells. The results represent the averages of 3 independent determinations performed in triplicate. *P<0.01 versus WT BM→WT+Ang II and Bcl10 KO BM→WT+Ang II, one-way ANOVA using Tukey’s post-hoc test, n=9 in WT BM→WT, n=7 in Bcl10 KO BM→WT and n=4 in WT BM→Bcl10 KO group.