Figure 5.

Contribution of Srr1 latch domain to GBS–fibrinogen interaction cellular attachment. A, Adherence of WT NCTC 10/84 or isogenic Δlatch to epithelial cells (MOI of 1) with or without the addition of exogenous fibrinogen (20 µg/mL). Data were analyzed by Student t test. *P < .05; **P < .005. B, Immunofluorescence staining of epithelial cells incubated with FLAG-tagged purified peptides (Srr1-BR and Srr1-BRΔlatch) probed with anti-FLAG antibody (red). Nuclei were visualized with DAPI (blue). (Magnification = 630×, scale bar = 20 µm.) Experiments were repeated at least twice with 4 replicates, and data from a representative experiment are shown. Abbreviations: BR, binding region; DAPI, 4’,6-diamidino-2-phenylindole; GBS, group B streptococcus; MOI, multiplicity of infection; Srr, serine-rich repeat; WT, wild type.