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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Mol Microbiol. 2014 Sep 15;94(2):367–382. doi: 10.1111/mmi.12770

Table 2.

Analysis of PE25–PPE41–EspG5 interactions in vitro.

PPE41 variant Interaction
WTa +
L125E
L125R
T129D/A130R
Q127I +
Q127I/F128N +
A130I +
Q127I/A130I +
Q127I/F128N/Q131P +
Q127I/F128N/A130I/Q131P +
A124F/L125F/Q127I/F128N +
A124F/L125F/Q127I/F128N/A130I/Q131P +
A124L +
A124L/L125F +
A124F/L125F +
A124W/L125F +

Interactions of PE25–PPE41 with EspG5 were analyzed using pull-down experiments presented in Fig. 3C, S8, S9 and S10.

a

WT — wild type.