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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: Structure. 2014 Sep 11;22(10):1489–1500. doi: 10.1016/j.str.2014.07.014

Figure 3.

Figure 3

MLKL(2-154) causes membrane leakiness. A. Liposome leakage assays monitoring the de-quenching of sulforhodamine B fluorescence as a function of time in the presence of difference concentrations of MLKL(2-154) (color-coded and indicated on the right in μM concentrations). The protein was added after 200 s. The data were normalized with the fluorescence observed after adding detergent, and setting at zero the fluorescence right before protein addition. B. Plot of the fluorescence intensity observed after 1700 s (expressed as % of the maximum) as a function of MLKL(2-154) concentration. The curve shows the fit to a Hill equation, which yielded an EC50 of 140 nM and a Hill coefficient of 1.5.