. Author manuscript; available in PMC: 2015 Oct 7.

Published in final edited form as: Structure. 2014 Sep 11;22(10):1489–1500. doi: 10.1016/j.str.2014.07.014

Table 2.

Fluorescence changes upon liposome binding for NBD labels placed at different positions of MLKL(2-154)

Residue	λ_max - lipids^a	I_max - lipids^b	λ_max + lipids^a	I_max + lipids^b	r^c
T9C	548	0.32	537	1.34	4.2
V13C	547	0.24	532	1.20	5.0
H33C	546	0.31	534	1.39	4.5
M44C	551	1.10	536	1.56	1.4
T60C	548	0.29	539	1.05	3.6
A67C	557	0.72	536	1.22	1.7
C86	542	1.42	535	1.85	1.3
D100C	551	0.24	533	1.05	4.4
D107C	550	0.26	536	1.11	4.3
L114C	552	0.24	539	0.61	2.5
Q139C	551	0.18	546	0.12	0.7

In nm. Estimated error ± 2 nm for the NBD labels with stronger fluorescence intensities and ± 3 nm for those with smaller intensities.

In arbitrary units. Values represent averages from at least five measurements. Standard deviations range from 3% to 7%.

The ratio r is defined as (I_max + lipids)/(I_max - lipids).