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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: Cell Metab. 2014 Sep 25;20(4):593–602. doi: 10.1016/j.cmet.2014.08.012

Figure 1. Expression of FoxO in diabetic islets and generation of triple FoxO knockouts in β-cells.

Figure 1

(A–B) mRNA levels (arbitrary units, AU) and Western blot analysis of the three FoxO isoforms in islet extracts of 24-week-old db/db, WT (C57BL/6) and KO (each islet extract was prepared with pooled islets from 2–3 mice per genotype).

(C–D) Representative islet immunohistochemistry using antibodies against FoxO1 (c, green), insulin (Ins, green in D), or glucagon (Gcg, red in D). Nucleus is stained blue with DAPI.

(E) Ratio of insulin- to glucagon-immunoreactive areas as determined by immunohistochemistry (n=4 mice per genotype, 6 sections per mouse).

(F) Morphometric analysis of β-cell mass in 12-week-old mice (n=4 mice per genotype, 4 sections per mouse).

(G–H) Quantification of insulin and glucagon content in acid-ethanol extracts from collagenase-purified islets (n=5 mice per group). All data are presented as means ± SEM. **p<0.01, ***p<0.001 by Student’s t test.