(A–B) Blood glucose and insulin levels in fasted, fed, and 4-hr-refed 12-week-old male mice (n=9 WT, 10 KO mice per genotype).
(C–E) Intraperitoneal glucose tolerance tests in male mice at different ages (n=12 WT, 8 KO mice per genotype at each age).
(F) Quantification of areas under the curve for the experiments shown in c-e.
(G–I) Hyperglycemic clamps in 24-week-old male mice, showing glucose infusion rates
(G), plasma insulin levels (I) and quantification of areas under the curve for the experiments shown in h and i (n=4 mice per genotype). All data are presented as means ± SEM. *p<0.05, **p<0.01, ***p<0.001 by two-way ANOVA.